不同园林废弃物处理方式下接种丛枝菌根真菌对刺槐幼苗生长及土壤结构特性的影响

Effects of Inoculating Arbuscular Mycorrhizal Fungi on the Growth and Soil Structure Characteristics of Robinia pseudoacacia under Different Garden Waste Treatment Methods

  • 摘要: 以刺槐(Robinia pseudoacacia)幼苗为研究对象,探究不同园林废弃物添加方式下接种丛枝菌根真菌(AMF)对刺槐生长、光合作用以及根际土壤结构特性的影响。在园林废弃物不添加(CK)、覆盖(WM)、掺入(WI)和氨化园林废弃物掺入(AWI)4种方式下分别接种AMF和不接种(N-AMF)幼套近明球囊霉(Claroideoglomus etunicatum)进行对比研究。结果表明:(1)不同园林废弃物添加方式对AMF菌根侵染率、孢子数、侵入位点数和泡囊数具有显著影响。与CK相比,WM、WI和AWI均有助于增加AMF菌根侵染率和孢子数量。CK和WM侵入位点数和泡囊数无显著差异,AWI菌根侵染率、孢子数、侵入位点数和泡囊数最高。(2)与CK相比,WM、WI和AWI均不同程度地促进刺槐幼苗生长,其中AWI下AMF处理刺槐幼苗株高、基径、相对生长速率和根冠比相比于N-AMF处理分别提高12.9%、4.6%、5.0%和20.9%,刺槐幼苗叶片叶绿素含量、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和水分利用效率(WUE)分别提高11.2%、18.9%、15.0%、6.4%和27.9%。(3)WM、WI和AWI对刺槐幼苗土壤理化性质的影响各不相同。AWI下土壤有机碳(SOC)、全氮(TN)、铵态氮(NH4+-N)、速效磷(SP)和速效钾(SK)含量最高,硝态氮(NO3--N)含量最低;AWI下AMF处理SOC、NH4+-N、NO3--N、SP和SK含量较N-AMF分别增加33.8%、7.8%、27.2%、8.3%和8.3%,TN含量无显著差异,土壤过氧化氢酶(S-CAT)、土壤蔗糖酶(S-SC)、土壤脲酶(S-UE)和土壤碱性磷酸酶(S-ALP)活性分别增加7.9%、14.5%、15.2%和23.4%。(4)废弃物添加方式对土壤水稳性团聚体的影响因其直径不同而异,同一添加方式下接种AMF整体促进了刺槐幼苗土壤水稳性团聚体的形成,但对直径<0.25 mm的团聚体含量无显著影响。AWI添加方式下接种AMF是促进刺槐幼苗生长及改善土壤结构特性的有效举措。

     

    Abstract: Taking Robinia pseudoacacia as the research object, this study explores the effects of inoculating arbuscular mycorrhizal fungi (AMF) on the growth, photosynthesis, and rhizosphere soil structure characteristics of R. pseudoacacia under different garden waste treatment methods. Under four different addition methods, including no addition of garden waste (CK), coverage of garden waste (WM), addition of garden waste (WI), and addition of ammoniated garden waste (AWI), AMF inoculum of Claroideoglomus etunicatum and non-inoculated control (N-AMF) were applied, resulting a total of eight treatments. The results show that different methods of adding garden waste had a significant impact on AMF mycorrhizal infection rate, spore count, invasion site count, and vesicle count. Compared with CK, WM, WI, and AWI addition methods all significantly increased AMF mycorrhizal infection rate and spore count, whereas there was no significant difference in invasion site count and vesicle count between CK and WM. Among all waste addition methods, AWI had the highest mycorrhizal infection rate, spore count, invasion site count, and vesicle count. Compared with CK, WM, WI, and AWI all promoted the growth of R. pseudoacacia to varying degrees. Under the addition of AWI, the height, base diameter, relative growth rate, and root-shoot ratio of R. pseudoacacia treated with AMF increased by 12.9%, 4.6%, 5.0%, and 20.9%, respectively. The chlorophyll content, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), and water use efficiency (WUE) of R. pseudoacacia leaves increased by 11.2%, 18.9%, 15.0%, 6.4%, and 27.9%, respectively, compared to N-AMF treatment. The effects of WM, WI, and AWI addition methods on the physicochemical properties of R. pseudoacacia soil are different. Under different waste addition methods, the AWI addition method has the highest soil organic carbon (SOC), total nitrogen (TN), ammonium nitrogen (NH4+-N), available phosphorus (SP), and available potassium (SK) content, while the nitrate nitrogen (NO3--N) content to be the lowest. There is no significant difference in TN content between AMF treatment and N-AMF treatment under AWI addition method. Compared to N-AMF treatment, the SOC, NH4+-N, NO3--N, SP, and SK content of AMF treatment increased by 33.8%, 7.8%, 27.2%, 8.3%, and 8.3%, respectively. The activities of soil peroxidase (S-CAT), amylase (S-SC), soil urease (S-UE), and soil alkaline phosphatase (S-ALP) increased by 7.9%, 14.5%, 15.2%, and 23.4%, respectively. The effect of waste addition methods on soil water stable aggregates varies with different diameters. Under the same addition method, inoculation with AMF promoted the formation of soil water stable aggregates in R. pseudoacacia as a whole, but had no significant effect on the content of aggregates with diameters < 0.25 mm. The conclusion is that the addition of ammoniated garden waste and inoculation with AMF is an effective measure to promote the growth of R. pseudoacacia, improve soil structure characteristics and effectively utilize garden waste.

     

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