Abstract: The biodiversity and population dynamics of amphibians are the basis of amphibians conservation. During the period of 2014-2018, amphibian species composition and population dynamics in Leigongshan National Reserve of Guizhou Province were investigated by using the transect sampling method. The results show that: (1) a total of 28 species and subspecies were found in the survey which belonging to 2 orders, 8 families and 16 genera, including 2 endangered species, Vibrissaphora leishanensis and Quasipaa boulengeri, 13 species recorded only in China and 4 species recorded only in Guizhou Province; (2) Megophrys leishanensis, Megophrys spinata, Odorrana huanggangensis, Hylarana guentheri, Nidirana leishanensis and Paramesotriton caudopunctatus were the dominant species in the reserve; (3) more amphibians were observed in June than in August and October; (4) the number of species and individuals of Amphibians at altitudes of 800- < 1 200 m were higher than those at other altitudes; (5) the number of species and individuals observed over the five years were with varying degrees of fluctuation and all bottomed out in 2018.