粪便中病原微生物快速检测方法研究进展

    Research Progress on Rapid Detection Methods for Pathogenic Microorganisms in Faeces

    • 摘要: 近年来国内乡村生态环境的保护和改善受到高度关注,其中厕所粪便的无害化处理和资源化利用是乡村居住环境改造的重点之一。粪便中的病原微生物可通过污染食物或水源感染人类,对公共健康和生态环境产生很大危害,因此针对粪便中病原微生物的快速检测方法的研究至关重要。该文综述了目前粪便中病原微生物快速检测方法的研究进展,特别是核酸检测法、免疫学检测法和生物传感器检测法这3类新兴的病原微生物快速检测方法。核酸检测法通对目标序列进行放大扩增,可以检测目标病原体的特定基因,检测准确度高;免疫学检测法利用抗原-抗体杂交反应,据此开发的试剂盒能够快速检测目标物;生物传感器检测法主要依靠光学和电化学分析技术,样品制备简单,能够实现检测系统的微型化、模块化和集成。文中对比了几种方法的检测限和检测时间,并对其优缺点和实际应用潜力进行了分析讨论。

       

      Abstract: In recent years, the protection and improvement of the rural ecological environment in China have received great attention. The harmless treatment and utilization of toilet faeces is one of the focuses of rural residential environment improvement. Pathogenic microorganisms in toilet faeces can infect human by polluting food or water, which brings great threat to public health and ecological environment. Therefore, research on rapid detection methods for pathogenic microorganisms in faeces is needed. This article reviews the recent research progress of fast detection methods for pathogenic microorganisms in faeces, which covers three emerging detection methods including nucleic acid detection method, immunological detection method and biosensor detection method. Nucleic acid-based detection methods amplify the target sequence, which can detect the specific genes of target pathogen with high detection accuracy. Immunological-based detection methods use antigen-antibody hybridization to develop detection kits that can quickly detect targets. Biosensor-based detection methods rely on optical and electrochemical analytical principles. It has simple sample preparation procedure, and the detection system can be miniaturized, modularized, and is integrable. This article compares the detection limit and detection time of these methods, and discusses their advantages, limitations, and practical application potentials.

       

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