Abstract: In recent years, the number of threatened amphibians species have increased due to the rapid growth of the habitat loss, fragmentation and degradation. In order to clarify the current resources and habitat use of amphibians in Poyang Lake National Nature Reserve, and to make reasonable suggestions for the amphibian protection in the reserve, the line-transect method was used to determine amphibian species composition, number and diversity in April, June and August 2018. The results show that total of eight species which belonging to 1 order, 4 families were found in the reserve, including 1 national class Ⅱ protected species and 5 species that belong to state protected "beneficial or important economic or scientific valuable terrestrial wild animals". Hong Kong Rice-paddy Frog (Fejervarya multistriata) is the dominant species in the reserve (3 432 individuals which accounted for 64.93% of the total). There were no significant differences in amphibians species composition, quantity and diversity among different months, and there were significant differences in the amphibians quantity and Pielou evenness index among the three habitats. It could be concluded that amphibians have different distribution regions in the reserve due to their selectivity to habitat and difference of niche breadth. By this study the resource status and species diversity of amphibians in the reserve have basically been exploided, and on this basis, the threatened extrinsic factors on local amphibians have been analyzed, and reasonable suggestions for the protection and management to amphibians are put forward.