周军英, 王长永, 续卫利. 温度、水分和盐度对转基因耐草甘膦大豆种子萌发和幼苗生长的影响[J]. 生态与农村环境学报, 2006, 22(2): 26-30.
    引用本文: 周军英, 王长永, 续卫利. 温度、水分和盐度对转基因耐草甘膦大豆种子萌发和幼苗生长的影响[J]. 生态与农村环境学报, 2006, 22(2): 26-30.
    ZHOU Jun-Ying, WANG Chang-Yong, XU Wei-Li. Comparison Between Transgenic Soybean and Non-Transgenic Soybean in Resistance to Stresses[J]. Journal of Ecology and Rural Environment, 2006, 22(2): 26-30.
    Citation: ZHOU Jun-Ying, WANG Chang-Yong, XU Wei-Li. Comparison Between Transgenic Soybean and Non-Transgenic Soybean in Resistance to Stresses[J]. Journal of Ecology and Rural Environment, 2006, 22(2): 26-30.

    温度、水分和盐度对转基因耐草甘膦大豆种子萌发和幼苗生长的影响

    Comparison Between Transgenic Soybean and Non-Transgenic Soybean in Resistance to Stresses

    • 摘要: 为了探明转CP4-EPSPS(5-烯醇丙酮酸莽草酸-3-磷酸合成酶)基因是否会对大豆种子及幼苗的抗逆性产生影响,进而为转基因耐草甘膦大豆的环境安全评价提供科学依据,通过室内试验研究了温度、水分和盐度对转基因耐草甘膦大豆ARG、受体大豆n-ARG及我国传统栽培大豆苏豆3号种子萌发与幼苗生长的影响。结果表明:在种子萌发阶段,ARG对高温胁迫和水分胁迫的抗性显著低于n-ARG和苏豆3号;ARG对盐分胁迫的抗性与n-ARG没有显著差异,但抗性明显弱于苏豆3号。幼苗生长阶段,在温度胁迫(高温40℃,低温15℃)和水分胁迫(渍水和干旱)条件下,ARG在株高增长量和生物量方面都没有表现出优势,相反,在某些方面反而抗性更弱。例如,在轻度干旱胁迫(相对含水量55%)条件下,ARG地上部干重明显小于n-ARG。ARG对盐分胁迫的抗性也弱于n-ARG及我国传统栽培大豆苏豆3号。总之,与非转基因受体大豆n-ARG和我国传统栽培大豆苏豆3号相比,转基因耐草甘膦大豆ARG在温度、水分和盐度逆境条件下的竞争性和入侵性不强。

       

      Abstract: To ascertain whether transgenic engineering will affect resistance of seeds and seedlings to stresses,and consequently provide first-hand scientific information for environmental safety assessment of transgenic soybean,comparative studies were conducted in laboratory and greenhouse on transgenic soybean ARG,it′s counterpart n-ARG and Su 3 a traditional soybean cultivar in China.Results show at the germination stage,ARG was much weaker than n-ARG and Su 3 in resistance to high temperature and water stress,and similar to n-ARG but weaker than Su 3 in resistance to salt stress.At the seedling stage,given temperature stress(high-40 ℃,low-15 ℃)and water stress(flood and drought),ARG showed no advantage in height and biomass,and on the contrary,some disadvantages in certain conditions.For example,under a slight drought(relative water content 55%),ARG was obviously less than n-ARG in dry weight of the overground part,and also weaker than n-ARG and Su 3 in salt tolerance.The research shows the herbicide-tolerant transgenic soybean ARG is rather weak in competitiveness and invasiveness under stress conditions.

       

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