Abstract: Agar,gelatin and gelatin-agar were used as carriers in an experiment to immobilize cellulase produced by actinomycete 2235 that is capable of decomposing cellulose.Activity of the cellulases fixed with different carriers on different substrates,CMS-Na and filter paper separately,was measured to determine which one was the best carrier.Results show that gelatin is the worst while gelatin-agar the bese.Activity of the cellulose immobilized with gelatin-agar on filter paper and on CMCase could be as high as 3317 and 8868 nakat·mL^-1,respectively.