盐京九号水稻秸秆对铜绿微囊藻(Microcystis aeruginosa)的抑制作用

    Inhibitory Effect of Rice Straw (Yanjing 9) on Microcystis aeruginosa

    • 摘要: 以盐京九号水稻为材料,研究经高温灭菌或物理粉碎后的水稻秸秆微细颗粒对铜绿微囊藻(Microcystis aeruginosa)生长的抑制及其作用机制。结果表明,当藻液中水稻秸秆添加量≥10g·L-1时,高温灭菌与未灭菌的水稻秸秆同样能抑制微囊藻生长。灭菌稻秆试验组藻细胞的呼吸速率和SOD活性都呈现出先上升后迅速下降的趋势;通过有机溶剂(石油醚、乙酸乙酯和乙醚)萃取稻秆水浸液,用滤纸片法在固体培养基上所做的抑藻试验结果表明,乙醚提取液有明显的抑藻圈。提示抑藻效应是水稻秸秆中某些化学物质溶出而产生的作用,与微生物的活动无关。进一步分离鉴定乙醚提取液结果显示,浸提液中有机酸类和酚类物质有显著的抑藻效应。水稻秸秆微粒的粒径越小,产生的抑藻效应出现得越早,也越明显,这可能还与秸秆微粒的吸附作用有关。

       

      Abstract: Inhibitory effect of rice straw (Yanjing 9) on the growth of Microcystis aeruginosa and the inhibitory mechanism were studied. Results show that when 10 g·L-1 or more of rice straw was added to M. aeruginosa liquid,both sterilized and un-sterilized rice straws could inhibit the growth of M. aeruginosa. In the sterilized group,the respiratory rate and SOD activity evidently increased firstly and then declined rapidly. The result of filter paper tests on solid medium show that among the extractives extracted by different organic solvents (petroleum ether,ethyl acetate and ether) from the leaching liquor of sterilized rice straw,ether extracts could be observed to have inhibitory effects on M. aeruginosa. This indicates that the algae-inhibition effect was caused by some substances in rice straw itself,rather than by microorganisms. Further study show that some organic acids and phenolic compounds in the ether extracts could inhibit the growth of M. aeruginosa. In addition,the smaller the fragments of rice straw were,the more obvious the inhibitory effect was,which might be related to the adsorption effect of rice straw.

       

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