Abstract: Development of eco-tourism in nature reserves has brought in economic benefits,while bringing about negative impacts,such as environmental pollution and ecological damage,on the reserves,which is contrary to and even conflicts with the original intention of protecting nature reserves.Through the survey of the status of construction and management of the nature reserves in 2006 and a special questionnaire on eco-tourism in nature reserves issued and collected in 2008,problems existing in the eco-tourism in nature reserves were systematically discussed from the aspects of eco-tourism-related macroscopic decision-making and management,administrative structure of nature reserve,and relationships of nature reserves with neighboring communities,tourism operators,tourists,and eco-environment,and corresponding countermeasures have been brought forward.