Abstract: Effects of extraneous substrates on the process and rate of organic nitrogen mineralization in black soil,Northeast China was studied,using the Stanford and Smith mineralization and incubation method.Results show that addition of glucose inhibited mineralization process and reduced mineralization rate of soil organic nitrogen and promoted fixation of soil nitrogen,and that combined application of glucose and nitrogen demonstrated positive effect on sequestration of organic nitrogen.In conclusion,glucose could regulate mineralization of soil organic nitrogen,which is of important significance in improving nitrogen utilization efficiency and reducing nitrogen loss in farmland.