2,4’-DDT对斑马鱼生长、繁殖力及卵黄蛋白原的影响

    Impacts of 2,4’-DDT on Growth, Reproduction and Vitellogenin(Vtg) of Zebrafish

      摘要
    • 摘要: 以斑马鱼体内卵黄蛋白原（Vtg）作为雌激素污染物的生物标志物，比较研究了不同浓度2,4′-DDT对成年斑马鱼生长、发育、繁殖以及体内Vtg含量的影响。结果表明，当水环境中ρ（2,4′-DDT）为2和10 μg·L-1时，斑马鱼产卵量和受精率与空白和溶剂对照组相比均显著下降（P＜0.05）。当水环境中ρ（2,4′-DDT）为0.2、2和10 μg·L-1时，斑马鱼肝脏指数与空白和溶剂对照组相比显著升高（P＜0.05）；10 μg·L-12,4′-DDT处理组雄性斑马鱼体内Vtg含量显著高于空白和溶剂对照组（P＜0.05），而0.2、2和10 μg·L-12,4′-DDT处理组雌性斑马鱼体内Vtg含量均显著高于空白和溶剂对照组（P＜0.05），且随暴露浓度的增加而增大。SDS-PAGE检测及免疫印迹分析均显示雄鱼血液内出现Vtg特异条带，表明2,4′-DDT对斑马鱼具有雌激素效应。

       

      Abstract: Using zebrafish’s vitellogenin as a biomarker of estrogenic pollutants, a comparative study was carried out to determine impacts of 2,4 '-DDT, varying in concentration, on growth, development, reproduction and vitellogenin in vivo. Results show that in water containing 2 and 10 μg•L-1 of 2,4 '-DDT, the egg yield and egg fertilization rate of fish decreased significantly, as compared with the blank group (P<0.05). In water containing 0.2, 2, 10 μg•L-1 of 2,4 '-DDT, the fish increased significantly in its hepaticosomatic index (P<0.05); A significant increase was observed in vitellogenin level of male fish treated with 10 μg•L-1 2,4 '-DDT (P<0.05), and of female fish treated with 0.2, 2, and 10 μg•L-1 2,4 '-DDT (P<0.05). And the increase got more significant with the exposure increasing in concentration . SDS-PAGE and Western blotting analysis revealed that a Vtg fragment appeared in the blood of male fish, which indicates that the 2,4 '-DDT has estrogenic effects on zebrafish.

       

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