硒与富氢水配施对盐胁迫下绿豆幼苗生长及根际细菌群落结构的影响

    Effects of the Combined Application of Selenium and Hydrogen-enriched Water on the Growth and the Structure of Rhizosphere Bacterial Community of Mung Bean Seedlings under Salt Stress

      摘要
    • 摘要: 为探究硒与富氢水配施对盐胁迫下绿豆幼苗生长和根际土壤细菌群落结构的影响，以晋绿8号为试验材料进行盆栽试验，研究不同浓度硒(2.5、5.0和7.5 mg·kg-1)与富氢水(25%、50%和100%)配施对1.5 g·kg-1NaCl胁迫下绿豆幼苗生理生化指标及根际土壤细菌群落结构的影响。结果表明：(1)与对照相比，盐胁迫处理绿豆幼苗株高、根长、叶片叶绿素含量以及POD、CAT、SOD活性均受到显著抑制。(2)与盐胁迫相比，不同浓度硒与富氢水配施均能有效促进株高和根长，使叶片叶绿素含量增加，POD、CAT、SOD活性增强，丙二醛含量减少。(3)不同浓度硒与富氢水配施对盐胁迫下根际土壤细菌结构有一定影响，其中T8(7.5 mg·kg-1亚硒酸钠+50%富氢水)和T9(7.5 mg·kg-1+100%)处理则显著增加盐胁迫下根际土壤细菌群落的多样性和丰富度，ACE指数分别增加24.51%和19.67%，Chao1指数分别增加24.75%和19.97%。不同浓度硒与富氢水配施均能有效缓解绿豆幼苗盐胁迫，改善根际土壤细菌群落结构，其中，7.5 mg·kg-1亚硒酸钠与50%富氢水配施效果最佳。

       

      Abstract: To investigate the effects of combined application of selenium and hydrogen-enriched water on the growth of mung bean seedlings and the structure of soil bacterial community in rhizosphere under salt stress, Jinlü 8 was chosen as the experimental material for pot experiment in this study. The effects of the combined application of different concentrations of selenium (2.5, 5.0 and 7.5 mg·kg-1) and hydrogen-enriched water (25%, 50% and 100%) on the physiological and biochemical indexes of mung bean seedlings and the structure of soil bacterial community in rhizosphere under the stress of NaCl with 1.5 g·kg-1were studied. The results show that: (1) Compared with the control, the plant height, root length, and the activities of chlorophyll content, POD, CAT and SOD in the leaves of mung bean seedlings were significantly inhibited under salt stress. (2) Compared with salt stress, the combined application of different concentrations of selenium and hydrogen-enriched water was found to be effective in promoting the plant height and root length, increasing the chlorophyll content of leaves, enhancing the activities of POD, CAT and SOD, and reducing the content of malondialdehyde. (3) The combined application of selenium and hydrogen-enriched water with different concentrations had a certain effect on the structure of soil bacterial in rhizosphere under salt stress. T8 (7.5 mg·kg-1 + 50%) and T9 (7.5 mg·kg-1 + 100%) treatments significantly increased the diversity and richness of soil bacterial communities in rhizosphere under salt stress. The ACE index and the Chao1 index increased by 24.51%, 19.67% and 24.75%, 19.97%, respectively. The combined application of different concentrations of selenium and hydrogen-enriched water could effectively alleviate the stress of salt on mung bean seedlings and improve the structure of soil bacterial community in rhizosphere. The combined application of 7.5 mg·kg-1 sodium selenite and 50% hydrogen-enriched water had the best effect on alleviating salt stress in mung bean seedlings.

       

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