Abstract: Clarifying the coupling coordination relationship between rural revitalization and ecosystem services is not only an objective requirement for consolidating and expanding poverty alleviation achievements, but also has certain practical implications for implementing the concept of "Lucid Waters and Lush Mountains are Invaluable Assets" and promoting the realization of ecological product value. Taking Dingjie County in the Tibet Autonomous Region, which is located in a priority area for biodiversity conservation in southeastern Himalayas, as an example, this paper constructs a research framework for the coupling coordination degree of rural revitalization-ecosystem services from the three dimensions of industrial revitalization, ecological beauty, and people's prosperity, and analyzes the coupling relationship between the rural revitalization index and ecosystem service value of 10 townships in Dingjie County from 2000 to 2020. The results show that: (1) the rural revitalization index of four townships in Dingjie County is higher than the county average, and the rural revitalization index in 2010-2020 has increased significantly compared with 2000-2010. (2) The ecosystem service value (ESV) was higher in Chentang Township, Duobuza Township, and Qiongzi Township, and the decreasing trend of ecosystem service value in Dingjie County during 2010-2020 was significantly slower than that in 2000-2010. (3) In 2020, the coupling coordination degree of 5 townships in Dingjie County was higher than the county average, with the spatial distribution of coupling coordination degree being low in the center of the county and high in the north and south. Finally, countermeasures and suggestions were proposed from the perspectives of industry guidance, ecological security, and classified assistance. The research results can provide technical support for promoting regional ecological economic development and rural revitalization.