Abstract: Environmental protection has always been centered on the using of lake sediment as a resource. The sediment collected from Hulun Lake was used as the main substrate to demonstrate the effectiveness and efficiency of lake sediment resource utilization in ecological restoration, and the planting substrate was created by combining cow dung, sheep dung, and straw. The experiment included seven treatments: CK, TN1, TN2, TN3, TY4, TY5, and TY6. Physical properties such as germination rate and survival rate of forage, plant height, average root length, and chemical properties such as organic matter, nutrient content, and pH of the soil were examined to determine the impact of planting substrates on the growth and development of steppe forage. The results show that TN3 (sediment∶cow dung∶straw=2∶3.25∶2) and TY6 (sediment∶sheep dung∶straw=2∶3.25∶2) treatments had the best effects on forage growth, and that Agropyron mongolicum grew much effectively than Leymus chinensis. Leymus chinensis had 19.00% and 28.00% germination rates in TN3 and TY6, respectively, and Agropyron mongolicum had 73.00% and 72.00% germination rates in TN3 and TY6, respectively. Adding sheep dung planting base at the same proportion is more beneficial to the growth of forage than the other treatments. As for the other physical property indexes of forage and chemical property indexes of forage and planting substrates, TN3 and TY6 are higher than the other treatments with the same raw material. This indicates that the higher the content of sediment and straw in the planting substrates, the better the forage growth. The results of this research can provide technical support for the utilization of Hulun Lake′s sediment resources and the managment of sandy meadows.