Abstract: The structure and function of urban ecosystem and its internal relationship are constantly changing under the interaction of human and nature. The scientific nature of development planning and the waste of resources are the essential and difficult points of current urban ecological management. Clarifying the status and internal relationship of urban ecosystem services and its influencing factors is of great significance for optimizing urban ecological management and implementing green sustainable development. In this study, the temporal and spatial variation characteristics and trade-off synergy of six types of ecosystem services of Luoyang City in 2010 and 2019 were quantitatively evaluated, the influencing factors of spatial differentiation of service and trade-off intensity were analyzed, and the spatial distribution of comprehensive cold and hot spots of ecosystem services was identified. The results show that: (1) The spatial distribution of ecosystem services in Luoyang City was highly coupled with the landscape pattern. Soil conservation and water production changed significantly, and the change area was concentrated in the southern forest land. (2) There was a synergistic relationship among carbon storage, habitat quality, aesthetic landscape and soil conservation services, and the trade-off relationship exists between grain yield, water yield and the rest services. (3) The trade-off intensity of ecosystem services in Luoyang City was high in south and low in north. The high-intensity areas were dominated by woodland landscape. Slop, NDVI, altitude, GDP and precipitation had relatively high explanations for the spatial heterogeneity of trade-off intensity. (4) The cold and hot spots of ecosystem services were mainly distributed in the northwest and southeast of the city, respectively. Service types in overlapping service areas were mainly carbon storage, soil conservation and habitat quality. Systematic quantitative research on urban ecosystem services was helpful to the clarification of the advantages and disadvantages of urban ecological base, and to the optimization of the allocation of environmental resources and to provide scientific basis for maximizing ecological benefits.