Abstract: Pilot area of Shennongjia National Park (SNP) contains important habitat of Asiatic black bear (Ursus thibetanus) in central China, however, there is a lack of research on the activity rhythm of this species in SNP, which is conducive for managers to make better management plan for conserving the species. Occurrence data of Asiatic black bear were collected from 772 camera trapping sites from 2018 to 2020 with 62 575 camera trapping nights. Date and time of photographs of Asiatic black bear were extracted to assess their activity rhythm. The chi-square test and one-way ANOVA test were used to analyze the behavior of black bear at different elevations and vertical migration in different seasons. Below are the research results: (1) The annual activity rhythm of Asiatic black bears in SNP is unimodal, with the highest activity intensity occurring in summer and autumn. The hibernation period of black bear lasts from late December to mid-April of the next year for about 3 and a half months. (2) Daily activity rhythm of Asiatic black bear in SNP was bimodal, and the intensity of diurnal activity was higher, showing obvious diurnal pattern. (3) Asiatic black bear of SNP was most active between the altitude from >1 000 to 2 600 m. (4) There were significant differences in the range of elevations for the activity of Asiatic black bear in SNP in different seasons, which showed obvious vertical migration behavior of the species. (5) The lowest photograph rate of Asiatic black bear in SNP occurred between the altitude of >1 600-1 800 m, as the length of highway and the number of settlements in this elevation section ranked the second and first in SNP, respectively, indicating that human activities had a great influence on Asiatic black bear activities. The findings on the activity rhythm of the Asiatic black bears will provide important basic data and scientific support for the conservation and management of Asiatic black bear population and mitigation of human-bear conflict in SNP.