Abstract: Identifying the urban-rural gradient of supply-demand matching of ecosystem services is the premise of overall promotion of urban-rural ecological construction, and is of great significance for the coordinated optimization of urban-rural residents′ well-being. Taking the main area of Nanchang city as an example, the paper evaluated the spatial characteristics of supply and demand by using the relative index of ecosystem services supply and demand, classified the supply and demand status by using the method of natural discontinuity, and stacked them yielding a variety of supply and demand matching types. The urban-rural gradient types were identified by combining landscape structure analysis and spatial location. The results show that: The areas with high and medium demand for ecosystem servicesare mainly located in urban area, while the areas with high and medium supply are mainly located in rural area. There are 6 matching types of supply-demand with different landscape structure and spatial location. There are six types of urban-rural gradient in the study area: urban inner circle, urban middle circle, suburban, agricultural, semi-ecological and ecological villages. There are four types of supply-demand matching types of ecosystem services in the suburban and three types of supply-demand matching types in the rural area. Urban-rural gradient of supply-demand matching of ecosystem services are influenced by distance gradient and landscape gradient, and the spatial structure show a circular layer, which is divided into four layers. In general, from the urban to the rural end, demand gradually decreases and supply gradually increases. The research proposes different corresponding regulatory strategies and specific measures for each gradient. Based on the perspective of gradient integration, the ecological integration path is proposed to promote the complementarity and flow of urban-rural supply and demand. The results provide useful references for urban-rural ecosystem service management and regional integrated development.