Abstract: Land surface temperature (LST) and vegetation coverage (VC) are important indicators of global eco-environmental changes. The spatial coupling seasonal differentiation of LST and VC can provide theoretical support for eco-environmental research. Based on MODIS images, this study used ArcGIS, MRT and GeoDa softwares to analyze variations in vegetation coverage and land surface temperature, and to explore the spatial coupling seasonal differentiation in the Three Gorges Reservoir area. The results show that the spatial distribution and seasonal variation of VC and LST in the Three Gorges Reservoir area are different. From the perspective of spatial distribution, the high value areas of LST are concentrated in the tail area of the reservoir, and the low value areas are distributed in the steep slope areas in the north and middle south of the reservoir. Compared with LST, VC showed the opposite distribution trend. Through the univariate linear regression and spatial autocorrelation analysis, VC and LST showed negative correlation in the Three Gorges Reservoir area, and the correlation between them is different among the four seasons. The significance of negative correlation between VC and LST in summer, spring, winter and autumn decreases in turn. The overall coupling effect of VC and LST in the Three Gorges Reservoir area is excellent. The coupling effect of seasonal change in the Three Gorges Reservoir area is in the order of autumn-winter > winter-spring > summer-autumn > spring-summer. Among the four seasons, the coupling degree within 0.8, 1 had the largest proportion of LST lag grid, followed by the synchronous type of VC and LST, and the proportion of VC lag grid was the smallest. The research results can further help to understand the changes of ecological environment in the Three Gorges Reservoir area, and can also provide a theoretical basis for the implementation of better ecological environment construction and protection in the Three Gorges Reservoir area.