Abstract: Amphibians are among the world′s most threatened species, and the amphibian monitoring could provide the basic information for their protection. To find out the temporal and spatial pattern of amphibian diversity in Fujian Junzifeng National Nature Reserve, 12 line transects that distributing in three different habitats (stream, paddy field and artificial water channel) were selected, for the investigation of environmental data, species and number of amphibians in April, June and August 2018. Then nine diversity indexes were calculated to examine the difference of amphibian diversity in different habitats and different seasons. The results show that: (1) a total of 2 124 amphibians belonging to 22 species, 17 genera, 7 families and 1 order were observed; (2) Fejervarya multistriata, Microhyla heymonsi, Nidirana adenopleura, and Odorrana huanggangensis were the dominant species in all the line transects, and Hoplobatrachus chinensis, the national class Ⅱ protected animal, had been recorded in all seasons; (3) the Pearson correlation analysis show that air temperature, water temperature and humidity positively affected species richness, relative species abundance, and Shannon-Wiener index; (4) species richness, relative abundance, Shannon-Wiener index, Margalef index, F index, and G index in June were significantly higher than those in April, but only species richness, relative abundance, and G index were higher in August than those in April; (5) the G-F index was not influenced by habitat type and observation time.