Abstract: Clarifying the relationship and interaction mechanisms of multiple ecosystem services can effectively improve the sustainable supply capacity of ecosystems. In this paper, 6 typical ecosystem services of Nanning City were estimated by InVEST model, the ecological service trade-off and synergy were analyzed by Moran′s I index, and the different ecosystem service clusters were recognized by principal component factor analysis and clustering method. The results show that: (1) The patterns of 6 typical ecosystem services in Nanning present significant spatial heterogeneity and is high in suburban area but low in urban area, except for water yield. (2) There is synergy relationship between support and adjustment services, but both synergy and trade-off relationship are existing between supply and other services. (3) The ecosystem services are affected by common driving factors revealed by principal component factor 1 and 3, and its mutual inhibition are represented by principal component factor 2. (4) The following 5 typical ecosystem service clusters were discovered in Nanning by employing cluster analysis: the mountain ecological balance area, forest ecological conservation area, urban ecological fragile area, hilly cultivated area, and valley cultivated area. This research results can provide scientific basis for the land use optimization and the ecological health and sustainable development in Nanning City.