Abstract: In order to study the odor problems of slow-flow water bodies in rural areas, flavor profile analysis (FPA) was used to analyze the types and intensity of the odor in rural slow-flow water body in a rural area of northern Jiangsu Province. The main odorous compounds in slow-flow water bodies in this area were identified by gas chromatography-mass spectrometry (GC-MS). The selective ion mass(SIM) monitoring mode detection methods for several typical odorant substances were established to detect the concentration of typical odorant substances in slowly flowing water and to analyze the seasonal and regional variation rules. The results show that:(1) the main odor types in the slow-flowing water in this area were mud, fish, putrefaction, mildew and grass, with occasional chemicals. The odor intensity of all water sample was the strongest in winter (≥ 8.5), and maintained around 4-8 in the rest of the seasons; (2) The odorant substances in the slow-flow water body were mainly dimethyl disulfide, dimethyl trisulfide and 1-methylnaphthalene, with a small amount of dimethyl isoborphenol and geosmin, and some water samples also contained butylamide; (3) The seasonal variations of odorant substances in slow-flow water bodies were quite different, and the peak concentration of odorant substances appeared in different seasons.