Abstract: The method of emission factors was adopted to estimate and analyze the distribution characteristics of ammonia emissions of livestock and poultry breeding industry in the Yangtze River Delta (YRD). The livestock ammonia emissions of 16 cities in the YRD in 2015 were estimated based on the activity data and emission factors of various ammonia sources. Results show that the livestock ammonia emissions in the YRD reached 214.5 kt in 2015, of which 14.03% were from non-scale, and 85.97% were from intensive cultivation. The ammonia emissions from pigs, sows, meat birds, egg birds, sheep and cows accounted for 41%, 2%, 27%, 15%, 12% and 3%, respectively. Nantong and Taizhou are the geographical centers of ammonia emission in the YRD, and the spatial distribution of ammonia emission tended to be high in the north and low in the south. The three cities which had higher intensity of ammonia emission are Nantong, Changzhou and Taizhou, whose intensity of ammonia emission was 5.47, 5.20 and 4.04 t·km^-2, respectively. Obvious monthly characteristics of ammonia emission coefficient were investigated and it was large in summer (July and August) and small in winter (December and January).