Abstract: To clarify the influence mechanism of reservoir sediment overlying water temperature, pH, dissolved oxygen, water dynamic water environment change on sediment nitrogen release intensity, sediment slobber samples were collected from Inner Mongolia Xilinguole League river reservoir and 4 factors and 5 levels of single factor release nitrogen simulation tests in chamber were designed. The results showed that:(1)the nitrogen release intensity of sediment was positively correlated with temperature. The total nitrogen release rate of sediment at 25℃ was 1 606.40 mg·m^-2, which was 1.9 times of the total nitrogen release rate under the condition of 5℃. Under the condition of partial acid or partial alkalinity, the total nitrogen release of sediment was also favorable. Under the neutral condition, the nitrogen release intensity of sediment was the weakest, and the fitted curve of nitrogen release intensity with pH showed an upward parabola. Under high intensity disturbance (R=100 r·min^-1), the nitrogen release intensity of sediment was 1 469.5 mg·m^-2, which was 2.1 times of the nitrogen release intensity under the static condition. Under the condition of low dissolved oxygen concentration or anoxic condition, the nitrogen release intensity of sediment is greater, and aerobic condition will inhibit the release of nitrogen in sediment. (2) A variety of linear function of nitrogen release intensity of environmental factors and sediment regression showed that sediment nitrogen release intensity and temperature, pH and hydrodynamic environment factors were the optimal quadratic function curve fitting, and dissolved oxygen in cubic function optimal curve fitting.