Abstract: The ecological security of urban landscape pattern is the pre-condition for the sustainable development of urban agglomerations. With the aim to clarify the ecological security of landscape pattern of agglomerated cities in Guizhou Province, ENVI and ArcGIS were used to interpret for local land use type in 2017. Therein, the ecological source plots were identified from the analysis on importance of ecosystem services and ecological environment sensitivity. Based on the proportion of rocky desertification and land use type, the areal surface of the resistance to agglomerated cities was constructed. Furthermore, the buffering zone was detected, hence the ecological node can be identified to generate the corridor by analyzing the cost at distance and path. The landscape ecological security pattern in Central Guizhou urban agglomeration was then constructed. And the following results were obtained. The low level security pattern in the landscape ecological security pattern in Central Guizhou urban agglomeration was 30 354.95 km², accounting for 56.42% of the total area, the middle level occupied 35 132.14 km², accounting for 65.30% of the total area, and the high level occupied 39 780.76 km², accounting for 73.94% of the total urban area. As the protection of the large area of Central Guizhou urban agglomeration need to be strengthened, attention should be paid to urban construction planning, so as, not to destroy the ecological environment in Central Guizhou urban agglomeration.