Abstract: The Chongqing Section of the Three-Gorge Reservoir Area with important eco-geographical significance was hence selected as subject of study. The method of statistics and network analysis was applied with the Landsat TM Remote Sensing Data of the region dated from 2000 to 2015 to study variation of the landscape pattern and ecosystem service value from the spatio-temporal aspect and their relationship. Results show:(1) On the whole, cultivated land and forest land still accounted for a large portion, while the other fractions of landscape, like grassland, water surface and construction land, only for small portions, separately. However, the fraction of construction land expanded the fastest; (2) Temporally speaking, during the period from 2000 to 2015 the indices of patch density and largest patch area decreased first and came backwards, and the maximum value of Landscape aggregation index and the minimum value of Shanon's diversity index appeared in 2010; and the total ecosystem service value exhibited a rising and then falling trend, and peaked in 2010; (3) Spatially speaking, the center of landscape fragmentation shifted gradually from the northeast and southeast of the region to well-built areas of the districts and counties and the main urban zone and the index of landscape integrity gradually increased in the northeast and southeast; The areas low in ecosystem service value were concentrated in the well-built areas, and the main urban zone was the lowest and the area on the west of the Yangtze River was lower than that on the east. All the findings demonstrate that a reasonable landscape pattern is of great significance to increasing ecosystem service value of a place like the Three-Gorge Reservoir Area (Chongqing Section).